Fig 1: Protein level validation for external cohorts. (A) Full tissue microarray scans with nuclei labeled with DAPI (blue), ALAD labeled with Alexa Fluor 488, FTCD labeled with Alexa Fluor 550, and PON1 labeled with Cy5. For better visualization, FTCD signals are converted to pseudo-color. (B) Distribution of the difference in staining intensities of ALAD, FTCD, and PON1 in HCC tissues compared with that in paired adjacent tissues. (****p < 0.001). (C–E) Representative images of multicolor IF staining in tissues. Adjacent tissues (C), triple-negative tumor tissues (D), and triple-positive tumor tissues with chaotic distribution (E). (F) K-M plot of the three-gene prognosis model in 136 patient external validation cohorts.